Challenge test identifies dietary health effects

A “high-fat challenge test” in the form of a fat-rich dairy shake given to overweight healthy men after consumption of a dietary supplement for 5 weeks, generates more and better information about the health effects of this supplement than other methods do.

TNO has demonstrated that a metabolomics & proteomics based high-fat challenge test applied in a dietary intervention, gives more additional information on the health status than measurements in a standard conditions. This extra information can be of value in the substantiation of health claims.

A fat-rich challenge test in overweight healthy men results in a significantly different health status after consuming a dietary supplement, which gives more information on the health status in a dietary intervention. Men in the intervention group revealed changes in protein and hormone metabolism and lowered inflammatory and oxidative stress response compared to the control group. The results of the study have been published in Metabolomics (Pellis et al. DOI 10.1007/s11306-011-0320-5).

During the study 36 overweight healthy men were given a daily dietary supplement for five weeks aimed at reducing oxidative stress and inflammation. The supplement comprised by TNO contains resveratrol, green tea extract, vitamin E, vitamin C, fish oil and tomatoes. Just before and 1, 2, 3, 4 and 6 hours after taking the high-fat challenge test, 231 plasma metabolites and proteins were measured with nutrigenomics technology. This enabled an accurate characterisation to be made of the response of the carbohydrate, protein and fat metabolism as well as processes like inflammation, the hormonal system and oxidative stress.
Challenge test

Ben van Ommen, Principal Scientist and System Biology programme director at TNO says, “Nowadays health is more seen as the ability of a person to adapt to changes (stressors). The body deals with stressors on a daily basis, especially in terms of metabolism (the digestion of meals) and inflammation (bacteria, viruses and exogenous compounds). By measuring the response to a stressor, we can get an indication of a person’s health.”


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